摘要
选取套作和单作种植方式与野生商洛丹参比较,采用高效液相色谱法和硝酸铝-亚硝酸钠比色法检测其丹参酮、丹酚酸和丹参多糖及总黄酮含量,探讨不同种植方式对商洛丹参主要药用成分含量的影响。结果表明:商洛野生丹参中丹参酮、丹酚酸B、丹参多糖及总黄酮含量最高,分别达到0.925%、9.27%、3.14%和2.895 mg/g;其次是套作丹参S1和S2样品,其中S1含量依次为0.834%、7.07%、2.52%和2.666 mg/g,S2含量依次为0.848%、7.14%、2.53%和2.711 mg/g;单作丹参含量最低,但不同种植方式的丹参质量均达到国家药典标准。套作丹参质量优于单作,可见实施经济林下套作,既能减小与野生丹参药用成分含量差异,又能缓解土地资源紧张局面,本研究可为丹参规范化种植提供基础资料。
Abstract
Shangluo wild S. miltiorrhiza B. and Shangluo S. miltiorrhiza B. of intercropping and monocropping tillage were selected respectively as samples, and the contents of tanshinone, salvianolic acid, S. miltiorrhiza polysaccharides and total flavonoids were determined respectively by HPLC or colorimetry. The effects of different tillage patterns on the main medicinal components of Shangluo S. miltiorrhiza B. were discussed. The results showed that the contents of tanshinone, Salvianolic acid B, S. miltiorrhiza polysaccharides and the total flavonoids in Shangluo wild S. miltiorrhiza B. were the highest among the samples, and they respectively reached 0.925%, 9.27%, 3.14% and 2.895 mg/g; as for intercropping S. miltiorrhiza, the contents in S1 respectively reached 0.834%, 7.07%, 2.52% and 2.666 mg/g, and the contents in S2 respectively reached 0.848%, 7.14%, 2.53% and 2.711 mg/g; the minimus content was monocropping S. miltiorrhiza B, but the quality of S. miltiorrhiza B. in different tillage patterns all met the national pharmacopoeia standard. At the same time, the quality of intercropping S. miltiorrhiza B. is better than that of monocropping, and the implementation of economic forests intrecropping can not only reduce the differences in the content of medicinal components between artificial-cultivated and wild S. miltiorrhiza B, but relieve the shortage of land resources, both of which have instructional significance for the standardized cultivation of S. miltiorrhiza B..
关键词
丹参 /
套作 /
单作 /
药用成分
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Key words
S. miltiorrhiza B. /
intercropping /
monocropping /
medicinal components
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何念武,梁媛,彭晓邦,王新军.
人工种植和野生丹参药用成分含量的比较研究. 陕西师范大学学报(自然科学版). 2019, 47(4): 102-107
HE Nianwu, LIANG Yuan, PENG Xiaobang, WANG Xinjun.
Comparative study on the contents of medicinal components between artificial-cultivated and wild Salvia Miltiorrhiza. Journal of Shaanxi Normal University(Natural Science Edition). 2019, 47(4): 102-107
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参考文献
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脚注
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基金
陕西省青年科技新星项目(2014KJXX-79);商洛学院科研计划项目(17SKY-FWDF007);陕西省生物学优势学科建设专项资金。
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