在环脂肽NRPS基因C1、Te保守区各设计一对引物，利用PCR技术，从福建和陕西两地的土样中筛选携带环脂肽NRPS基因的荧光假单胞菌，运用SSCP、酶切分型的方法分析NRPS基因的多态性。结果显示，福建、陕西两地菌株的C1和Te保守区经SSCP电泳后所得的条带数均有3、5、7带型，且条带位置有差异。据统计，两地C1区均有10个分型，Te区总计10个分型。其中福建菌株Te区有9个分型，无SSCP-10型；陕西菌株Te区有8个分型，无SSCP-2、SSCP-4型。用限制性内切酶HinfⅠ和SphⅠ对NRPS基因Te区进行酶切，福建菌种Te区扩增产物的酶切产物分为15种，陕西菌种Te区的酶切产物分为11种。表明荧光假单胞菌NRPS基因具有多种形态，且不同地区的类型分布有所差异。相较之下，福建菌株的NRPS基因多样性更好，更有可能获得活性较强或产新型环脂肽的菌株。

The primers at C1 and Te region of NRPS gene were designed respectively and PCR technology was employed to find Pseudomonas fluorescens carrying cyclic lipopeptide genes from soil samples of Fujian and Shaanxi province.Then SSCP and Restriction endonuclease method were carried out to analyze the diversity of NRPS genes.Results show that the C1 and Te regions of Fujian and Shaanxi bacterial strains display 3,5,7 three kind bands by SSCP electrophoresis and the positions of bands are different. C1 region has 10 types,and Te region has 10 types in total. Among them Fujian strains have 9 types except SSCP-10,while Shaanxi strains have 8 types except SSCP-4 and SSCP-2.Cutting Te region of NRPS genes by two different restriction enzymes HinfⅠ and SphⅠ,the digestion products of Fujian strains are divided into 15 kinds,while Shaanxi strains are divided into 11 kinds.It shows that NRPS gene has a variety of forms, and the types of distribution in different regions vary.In conclusion,the NRPS genes in Fujian strains have more diversity which suggests that these strains have more potential to produce new types of lipopeptide.