在金纳米粒子修饰石墨电极上，鲁米诺-过氧化氢-二茂铁羧酸体系的电化学发光有增敏作用．详细考察了在金纳米粒子修饰石墨电极上鲁米诺-过氧化氢-二茂铁羧酸体系的电化学发光行为，研究了影响发光信号的多种因素，优化了测定二茂铁羧酸的分析条件．结果表明，在2.5×10-9mol/L鲁米诺，1.0×10-3 mol/L过氧化氢，0.10 mol/L pH值为9.2 碳酸氢钠缓冲溶液中，以金纳米粒子修饰石墨电极为工作电极, 控制电位为+390 mV, 二茂铁羧酸浓度在1.0×10-9～1.0×10-6 mol/L范围内与电化学发光强度呈良好的线性响应关系，检出限为7×10-10 mol/L(S/N=3)．

A highly sensitive electrogenerated chemiluminescence (ECL) method for the determination of ferrocenemonocarboxylic acid (FMC) was developed. It was found that the ECL intensity of luminol-H2O2-FMC system could be greatly enhanced at a graphite electrode (GE) modified with gold nanoparticles. The test conditions including the modified amount of gold nanoparticles on GE, working potential, the concentrations of luminol and hydrogen peroxide were optimized. The integrated ECL intensity at the applied potential of +390 mV (vs. Ag/AgCl, sat. KCl) in 0.10 mol/L carbonate buffer solution (pH 9.2) containing 2.5×10-9 mol/L luminol and 1.0×10-3 mol/L hydrogen peroxide linearly respond to the concentration of FMC in the range from 1.0×10-9 mol/L to 1.0×10-6 mol/L. The detection limit is 7.0×10-10 mol/L (S/N=3). The developed method has a potential application to the ECL immunoassay and DNA hybridization detection based on the FMC label.